| Intrument | Fluorescence microplate reader (Ex/Em=535 nm/590 nm) |
| Assay Time | 70 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Plant tissue |
| Detection Principal | Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Labware | Micropipettor, Incubator, Water bath, Centrifuge |
| Size | 96T |
| Sensitivity | 0.15 ?mol/L |
| Detection Range | 0.15-15 ?mol/L |
| Recovery Rate | 96 |
| Inter CV | 6.5 |