Intrument | Microplate reader (640-660 nm, optimum wavelength: 650 nm) |
Assay Time | 40 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,urine,tissue |
Synonyms | T-AA |
Detection Principal | Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Test tube, Micropipettor, Vortex mixer, Centrifuge |
Size | 96T |
Sensitivity | 3.03 mmol/L |
Detection Range | 3.64-100 mmol/L |
Recovery Rate | 102 |
Inter CV | 6.5 |