| Intrument | Microplate reader (550 nm) |
| Assay Time | 40 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | serum, plasma, animal tissue |
| Synonyms | ADA |
| Detection Principal | Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Micropipette, Centrifuge, Water bath/Incubator |
| Size | 96T |
| Sensitivity | 0.03 U/L |
| Detection Range | 0.03-99 U/L |
| Recovery Rate | 98 |
| Inter CV | 6 |