| Intrument | Microplate reader (450 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | cells |
| Synonyms | LDH |
| Detection Principal | Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm. |
| Labware | Microplate centrifuge, Micropipettor, Water bath |
| Size | 96T |