Intrument | Microplate reader (450 nm) |
Assay Time | 30 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | cells |
Synonyms | LDH |
Detection Principal | Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm. |
Labware | Microplate centrifuge, Micropipettor, Water bath |
Size | 96T |