Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

Cat No.:HR3BC1128
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  Product info

Detection Method: Colorimetric method

Storage : Reagent 1: 2-8?, Reagent 2: -20?
Product Details

Intrument Microplate reader(620-640 nm)
Assay Time 20 min
Assay TypeQuantitative
Sample TypeSerum,plasma
Synonyms ALB
Detection Principal Bromocresol green (BCG) is widely used as protein staining agent. BCG can combine the albumin in pH 4.0~4.2 to form an albumin-BCG complex. And the color changed from yellow to green. The depth of color is proportional to the concentration of albumin. The content of albumin in serum can be calculated indirectly by measuring the OD value at 630 nm.
ReagentsNormal saline (0.9% NaCl)
LabwareMicropipettor, Vortex mixer
Sensitivity 0.08 g/L
Detection Range 0.08-15 g/L
Recovery Rate 95
Inter CV 4.6

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