| Intrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Assay Time | 30 min |
| Validity | 3 |
| Assay Type | Quantitative |
| Sample Type | serum, plasma, wine |
| Detection Principal | Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm. |
| Labware | Micropipettor, Vortex mixer, Centrifuge, Incubator (37?) |
| Size | 96T |
| Sensitivity | 0.27 ?mol/mL |
| Detection Range | 0.27?17.0 ?mol/mL |
| Recovery Rate | 96 |
| Inter CV | 5 |