| Intrument | Microplate reader (562 nm) |
| Assay Time | 35 min |
| Validity | 1 year |
| Assay Type | Quantitative |
| Detection Principal | The BCA protein assay is a widely used method for protein quantification, utilizing bicinchoninic acid (BCA) for colorimetric detection. This technique combines the reduction of Cu²⁺ to Cu⁺ by proteins in an alkaline environment (the biuret reaction) with the formation of a purple-coloured complex resulting from the interaction of Cu⁺ with BCA. This complex has a maximum absorbance at 562 nm and exhibits a nearly linear response to protein concentrations across a broad range (0–2 mg/mL). Due to its high sensitivity and ease of use, the BCA protein assay is commonly adopted by laboratories and industries as a preferred method for protein quantification, alongside the Bradford assay. |
| Labware | Micropipettor, Centrifuge, incubator |
| Size | 2500T |
| Detection Range | up to 3 mg/ml |