| Intrument | Spectrophotometer(560 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,saliva,urine,hydrothorax,tissue,cells |
| Synonyms | SA |
| Detection Principal | Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm. When detect tissue and cell samples, the protein concentration of the sample needs to be detected in addition (E-BC-K165, E-BC-K168, E-BC-K318 are recommended). |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 100Assays |
| Sensitivity | 0.022 mmol/L |
| Detection Range | 0.022-7 mmol/L |
| Recovery Rate | 95 |
| Inter CV | 7.1 |