Sialic Acid (SA) Colorimetric Assay Kit

Sialic Acid (SA) Colorimetric Assay Kit

Cat No.:HR3BC1130
Product available

  Product info

Detection Method: Colorimetric method

Storage : Reagent 1: -20?, Reagent 2: 2-8?
Product Details

Intrument Spectrophotometer(560 nm)
Assay Time 60 min
Assay TypeQuantitative
Sample TypeSerum,plasma,saliva,urine,hydrothorax,tissue,cells
Synonyms SA
Detection Principal Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm. When detect tissue and cell samples, the protein concentration of the sample needs to be detected in addition (E-BC-K165, E-BC-K168, E-BC-K318 are recommended).
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Incubator, Vortex mixer, Centrifuge
Sensitivity 0.022 mmol/L
Detection Range 0.022-7 mmol/L
Recovery Rate 95
Inter CV 7.1

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