| Intrument | Spectrophotometer(620 nm) |
| Assay Time | 50 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Animal liver,muscle |
| Detection Principal | Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen. |
| Reagents | Normal saline (0.9% NaCl), Concentrated sulfuric acid |
| Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 100Assays |
| Sensitivity | 1.80 mg/g liver tiss |
| Detection Range | 1.80-180 mg/g liver |
| Recovery Rate | 101 |
| Inter CV | 7.3 |