Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

Cat No.:HR3BC1131
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Spectrophotometer(620 nm)
Assay Time 50 min
Assay TypeQuantitative
Sample TypeAnimal liver,muscle
Detection Principal Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
ReagentsNormal saline (0.9% NaCl), Concentrated sulfuric acid
LabwareMicropipettor, Incubator, Vortex mixer, Centrifuge
Sensitivity 1.80 mg/g liver tiss
Detection Range 1.80-180 mg/g liver
Recovery Rate 101
Inter CV 7.3

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