Intrument | Spectrophotometer(460 nm) |
Assay Time | 90 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum,plasma,milk,animal tissue,cells |
Synonyms | MPO |
Detection Principal | Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm. |
Labware | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
Size | 100Assays |
Sensitivity | 4.9 U/L |
Detection Range | 4.9-196.7 U/L |
Recovery Rate | 104 |
Inter CV | 9.8 |