Intrument | Spectrophotometer(520 nm) |
Assay Time | 60 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum,plasma,urine,tissue,cells |
Synonyms | ALP |
Detection Principal | Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Size | 100Assays |
Sensitivity | 0.2 King unit/100 mL |
Detection Range | 0.2-55.6 King unit/1 |
Recovery Rate | 99 |
Inter CV | 5.6 |