Intrument | Spectrophotometer(405 nm) |
Assay Time | 60 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,cell culture supernatant,tissue,cells |
Synonyms | H2O2 |
Detection Principal | Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Size | 100Assays |
Sensitivity | 1.5 mmol/L |
Detection Range | 1.5-150 mmol/L |
Recovery Rate | 98 |
Inter CV | 2.7 |