| Intrument | Microplate reader(600-620 nm,optimum wavelength: 610 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,urine,cell culture supernatant,tissue,cells |
| Synonyms | Ca |
| Detection Principal | Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715nm and calcium content can be calculated by measuring the OD value at 610 nm. |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-1.2 mmol/L |
| Recovery Rate | 99 |
| Inter CV | 8.5 |