Intrument | Spectrophotometer(554 nm) |
Assay Time | 30 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,urine |
Synonyms | D-aldopentose |
Detection Principal | D-xylose can produce furfural by dehydration in strong acid solution. The generated furfural reacts with Phloroglucinol to form pink compounds. The content of D-xylose can be calculated by colorimetric assay at 554 nm. |
Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer, Water bath |
Size | 100Assays |
Sensitivity | 0.007 mmol/L |
Detection Range | 0.007-4 mmol/L |
Recovery Rate | 103 |
Inter CV | 4.5 |