Intrument | Spectrophotometer(370 nm) |
Assay Time | 240 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,hydrothorax,cell culture supernatant,tissue |
Detection Principal | The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly. |
Reagents | Absolute ethanol, Ethyl acetate |
Labware | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
Size | 100Assays |
Sensitivity | 0.02 nmol/mgprot |
Detection Range | 0.02-10 nmol/mgprot |
Recovery Rate | 101 |
Inter CV | 8.6 |