| Intrument | Spectrophotometer(370 nm) |
| Assay Time | 240 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,hydrothorax,cell culture supernatant,tissue |
| Detection Principal | The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly. |
| Reagents | Absolute ethanol, Ethyl acetate |
| Labware | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Size | 100Assays |
| Sensitivity | 0.02 nmol/mgprot |
| Detection Range | 0.02-10 nmol/mgprot |
| Recovery Rate | 101 |
| Inter CV | 8.6 |