| Intrument | Fluorescence Microplate reader (Ex/Em=500 nm/525 nm), Fluorescence Spectrophotometer (Ex/Em=500 nm/5 |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Cell-based (quantitative) |
| Sample Type | Fresh tissue,cultured cells |
| Synonyms | ROS |
| Detection Principal | DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species. |
| Labware | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Size | 96T |