| Intrument | Spectrophotometer(520 nm) |
| Assay Time | 80 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Plant tissue,honey |
| Synonyms | Pro |
| Detection Principal | Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm. |
| Reagents | Acetic acid, Methylbenzene, Absolute ethyl alcohol |
| Labware | Water bath, Micropipettor, Vortex mixer, Balance, Centrifuge |
| Size | 100Assays |
| Sensitivity | 0.17 ?g/mL |
| Detection Range | 0.17-35 ?g/mL |
| Recovery Rate | 105 |
| Inter CV | 5.4 |