Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Cat No.:HR3BC1113
Product available

  Product info

Detection Method: Colorimetric method

Storage : Reagent 4: -20?, others: 2-8?
Product Details

Intrument Spectrophotometer(550 nm)
Assay Time 100 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,urine,cells,cell culture supernatant,tissue
Synonyms T-SOD
Detection Principal The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.
ReagentsNormal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Vortex mixer, Water bath, Centrifuge
Sensitivity 4.7 U/mL
Detection Range 4.7-166 U/mL
Recovery Rate 105
Inter CV 6.3

Write us

Superfast Delivery
Customer Support
Secure Online Payment
website designing in delhi website development company in delhi website developers in delhi freelancer website designer in delhi india best website development company in delhi