Intrument | Spectrophotometer(405 nm), Microplate reader(405 nm), Biochemistry analyzer(405 nm) |
Validity | 12 |
Assay Type | Quantitative |
Sample Type | Serum |
Synonyms | Na |
Detection Principal | Production of o-nitrophenol and galactose by o-nitrophenol-?-D-galactoside (ONPG) catalyzed by sodium dependent ?-D-galactosidase. The amount of o-nitrophenol is directly proportional to the concentration of sodium ion in the sample. The o-nitrophenol is yellow in alkaline environment. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly. |
Size | 200Assays |
Detection Range | 80-180 mmol/L |