| Intrument | Spectrophotometer (660nm) |
| Assay Time | 50 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Animal tissue |
| Synonyms | Ca2+-ATPase |
| Detection Principal | ATPase exists on the membrane of tissue cells and organelles. It is a kind of protease on the biological membrane which plays an important role in material transport, energy conversion and information transmission. Ca2+ which participates in the regulation of different enzyme systems and cell activities plays many important roles in cells. The flow of Ca2+ depends on the Ca2+ -ATPase on the cell membrane, and Ca2+ -ATPase consumes ATP to generate the energy needed for ion transport. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Test tube, Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Size | 100 assays |
| Sensitivity | 0.8 U/g wet weight |
| Detection Range | 0.8-41 U/g wet weight |
| Inter CV | 8.8 |