Intrument | Microplate reader(405-425 nm) |
Assay Time | 20 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,urine,saliva,tissue,cells |
Synonyms | T-AOC |
Detection Principal | The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), 80% Ethanol |
Labware | Micropipettor |
Size | 96T |
Sensitivity | 0.047 mmol/L |
Detection Range | 0.047-1.50 mmol/L |
Recovery Rate | 101 |
Inter CV | 4.1 |