Intrument | Spectrophotometer(240 nm?420 nm) |
Assay Time | 60 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Plant tissue |
Synonyms | POD |
Detection Principal | The peroxidase can catalyze the decomposition of H2O2 and produce water and oxygen. And oxygen oxidized pyrogallic acid to form yellow product. The activity of peroxidase can be calculated by measuring the absorbance at 420 nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Incubator, Vortex mixer, Micropipettor, Centrifuge |
Size | 100Assays |
Sensitivity | 0.5 U/mL |
Detection Range | 0.5-40 U/mL |
Recovery Rate | 98 |
Inter CV | 5.5 |