| Intrument | Microplate reader(500-520 nm) |
| Assay Time | 80 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,animal tissue,cells,cell culture supernatant |
| Synonyms | ALT,GPT,Glutamic-pyruvic transaminase |
| Detection Principal | ALT catalyze the amino conversion reaction between alanine and ?-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37?. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, Incubator, Multichannel pipette |
| Size | 96T |
| Sensitivity | 0.75 IU/L |
| Detection Range | 0.75-72.3 IU/L |
| Inter CV | 9 |