Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

Cat No.:HR3BC1156
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Microplate reader(500-520 nm)
Assay Time 80 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,animal tissue,cells,cell culture supernatant
Synonyms ALT,GPT,Glutamic-pyruvic transaminase
Detection Principal ALT catalyze the amino conversion reaction between alanine and ?-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37?. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Vortex mixer, Incubator, Multichannel pipette
Sensitivity 0.75 IU/L
Detection Range 0.75-72.3 IU/L
Inter CV 9

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