Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Cat No.:HR3BC1114
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  Product info

Detection Method: Colorimetric method

Storage : Reagent 3: -20?, others: 2-8?
Product Details

Intrument Microplate reader(440-460 nm, optimum wavelength: 450 nm)
Assay Time 30 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,hydrothorax,ascites,urine,cells,tissue
Synonyms T-SOD
Detection Principal The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
ReagentsNormal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Multichannel pipettor, Vortex mixer, Incubator
Sensitivity 0.2 U/mL
Detection Range 0.2 -14.4 U/mL
Recovery Rate 97
Inter CV 3.7

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