| Intrument | Spectrophotometer(290 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Plant tissue |
| Synonyms | APX |
| Detection Principal | Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Size | 100Assays |
| Sensitivity | 0.071 U/g tissue |
| Detection Range | 0.071-47 U/g tissue |
| Recovery Rate | 96 |
| Inter CV | 6.4 |