Ascorbate Peroxidase (APX) Activity Assay Kit

Ascorbate Peroxidase (APX) Activity Assay Kit

Cat No.:HR3BC1160
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Spectrophotometer(290 nm)
Assay Time 60 min
Assay TypeEnzyme Activity
Sample TypePlant tissue
Synonyms APX
Detection Principal Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareVortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Sensitivity 0.071 U/g tissue
Detection Range 0.071-47 U/g tissue
Recovery Rate 96
Inter CV 6.4

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