Intrument | Spectrophotometer(760 nm) |
Assay Time | 60 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Plant tissue |
Detection Principal | Under alkaline conditions, tungsten-molybdenum acid can be reduced by phenols and produce blue compounds, which has a characteristic absorption peak at 760 nm. The content of total phenols in sample can be calculated indirectly by measuring the absorbance at 760 nm. |
Reagents | 60% Ethanol |
Labware | Micropipettor, Vacuum dryer, Vortex mixer, Ultrasonic cell grinder, Crusher |
Size | 100Assays |
Sensitivity | 0.73 ?g/mL |
Detection Range | 0.73-150 ?g/mL |
Recovery Rate | 101 |
Inter CV | 2.5 |