| Intrument | Semi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate re |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue homogenate,cells,cell supernatant |
| Synonyms | NEFA |
| Detection Principal | NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly. |
| Reagents | Normal saline (0.9% NaCl) |
| Size | 96T |
| Detection Range | 0.01-3.0 mmol/L |