Intrument | Spectrophotometer(420 nm) |
Assay Time | 50 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,tissue,cells |
Synonyms | GSH |
Detection Principal | Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm. |
Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer |
Size | 100Assays |
Sensitivity | 0.26 mg GSH/L |
Detection Range | 0.26-122.8 mgGSH/L |
Recovery Rate | 102 |
Inter CV | 2.4 |