| Intrument | Spectrophotometer(420 nm) |
| Assay Time | 50 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue,cells |
| Synonyms | GSH |
| Detection Principal | Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm. |
| Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer |
| Size | 100Assays |
| Sensitivity | 0.26 mg GSH/L |
| Detection Range | 0.26-122.8 mgGSH/L |
| Recovery Rate | 102 |
| Inter CV | 2.4 |