Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit


Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

Cat No.:HR3BC1169
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Spectrophotometer(520 nm)
Assay Time 50 min
Validity6
Assay TypeQuantitative
Sample TypeSerum
Synonyms TIBC
Detection Principal The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).
ReagentsNormal saline (0.9% NaCl)
LabwareMicropipettor, Water bath, Vortex mixer, Centrifuge
Size50Assays
Sensitivity 0.03 mg/L
Detection Range 0.03-50 mg/L
Recovery Rate 100
Inter CV 4.7

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