| Intrument | Spectrophotometer(660 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Animal tissue,cells |
| Detection Principal | ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
| Size | 100Assays |
| Recovery Rate | 109 |
| Inter CV | 9.8 |