Intrument | Spectrophotometer(540 nm) |
Assay Time | 50 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,tissue |
Synonyms | TP |
Detection Principal | Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Incubator |
Size | 100Assays |
Sensitivity | 0.373 g/L |
Detection Range | 0.373-80 g/L |
Recovery Rate | 99 |
Inter CV | 2.6 |