| Intrument | Microplate reader(440 nm-480 nm,optimum wavelength: 460 nm) |
| Assay Time | 15 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | Cl |
| Detection Principal | Chloride ion in biological fluids are replaced by the mercury ions in mercury thiocyanate through ion replacement, which resulted in the formation of difficult-to-dissociate mercury chloride. The substituted thiocyanate ions were combined with ferric nitrate to form a red complex. The content of chlorine ion can be calculated indirectly by measuring the OD value at 460 nm. |
| Labware | Micropipettor, Tubes, Vortex mixer, Incubator, Centrifuge. |
| Size | 96T |
| Sensitivity | 1 mmol/L |
| Detection Range | 1.0-60 mmol/L |
| Recovery Rate | 105 |
| Inter CV | 6.4 |