| Intrument | Microplate reader(540-590 nm, optimum wavelength: 562 nm) |
| Assay Time | 40 min |
| Validity | 12 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,cell culture supernatant,tissue,cells |
| Synonyms | TP |
| Detection Principal | Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Vortex mixer, Micropipettor, Incubator |
| Size | 500Assays |
| Sensitivity | 0.0165 mg/mL |
| Detection Range | 0.0165-1 mg/mL |
| Recovery Rate | 100 |
| Inter CV | 4.5 |