Intrument | Microplate reader(540-590 nm, optimum wavelength: 562 nm) |
Assay Time | 40 min |
Validity | 12 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,cell culture supernatant,tissue,cells |
Synonyms | TP |
Detection Principal | Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Vortex mixer, Micropipettor, Incubator |
Size | 500Assays |
Sensitivity | 0.0165 mg/mL |
Detection Range | 0.0165-1 mg/mL |
Recovery Rate | 100 |
Inter CV | 4.5 |