| Intrument | Microplate reader(405-415 nm,optimum wavelength: 412 nm) |
| Assay Time | 100 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,animal tissue,red blood cells,cultured cells |
| Synonyms | T-GSH,GSSG |
| Detection Principal | GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle. |
| Reagents | PBS (0.01 M, pH 7.4), Absolute ethanol |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.36 ?mol/L T-GSH |
| Detection Range | 0.36-30 ?mol/L T-GSH |
| Recovery Rate | 97 |
| Inter CV | 3.9 |