Intrument | Spectrophotometer(370 nm) |
Assay Time | 50 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,,tissue |
Detection Principal | Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer |
Size | 100Assays |
Sensitivity | 0.94 ?g/mL |
Detection Range | 0.94-45 ?g/mL |
Recovery Rate | 100 |
Inter CV | 8.3 |