Intrument | Microplate reader(500-520 nm) |
Assay Time | 80 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum,plasma,animal tissue,cells,cell culture supernatant |
Synonyms | AST,GOT,Glutamic-oxalacetic transaminase |
Detection Principal | AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer, Incubator, Multichannel pipette |
Size | 96T |
Sensitivity | 1.1 IU/L |
Detection Range | 1.1-72.3 IU/L |
Inter CV | 6.8 |