| Intrument | Microplate reader(500-530 nm,optimum wavelength: 520 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,tissue,cells |
| Synonyms | ALP |
| Detection Principal | Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.13 King unit/100 m |
| Detection Range | 0.13-50 King unit/10 |
| Recovery Rate | 94 |
| Inter CV | 8.5 |