Intrument | Spectrophotometer(290 nm) |
Assay Time | 40 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Plant tissue |
Detection Principal | Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose. |
Reagents | PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer, 100? Water bath |
Size | 100Assays |
Sensitivity | 0.32 ?mol/mL |
Detection Range | 0.32-70 ?mol/mL |
Recovery Rate | 102 |
Inter CV | 8.5 |