| Intrument | Spectrophotometer(290 nm) |
| Assay Time | 40 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Plant tissue |
| Detection Principal | Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose. |
| Reagents | PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, 100? Water bath |
| Size | 100Assays |
| Sensitivity | 0.32 ?mol/mL |
| Detection Range | 0.32-70 ?mol/mL |
| Recovery Rate | 102 |
| Inter CV | 8.5 |