| Intrument | Microplate reader (550 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | 5?-NT |
| Detection Principal | 5'-Nucleotide enzyme (5'-NT, EC 3.1.3.5), full name is 5'-ribonucleotide phosphohydrolase, is a special phosphohydrolase that specifically hydrolyzes 5'-phosphoric acid attached to pentose in 5'-nucleotide. This enzyme is widely distributed in the cell membrane of various tissues of human and animal. Only the 5'-NT released by the tissue cells of the hepatobiliary system may enter the blood. Therefore, the source of serum 5'-NT has certain specificity, and the determination of serum 5'-NT has important value for the diagnosis of hepatobiliary diseases. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Pipettor, Water bath, Centrifuge |
| Size | 96T |
| Sensitivity | 28.0 U/L |
| Detection Range | 28.0-581 U/L |
| Recovery Rate | 105 |
| Inter CV | 6 |