Intrument | Microplate reader(590-600 nm) |
Assay Time | 20 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,saliva,urine,tissue,cells,cell culture supernatant |
Synonyms | T-AOC |
Detection Principal | Fe3+-TPTZ can be reduced by antioxidants and produce blue Fe2+-TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Balance, Incubator, Micropipettor |
Size | 96T |
Sensitivity | 0.049 mmol/L |
Detection Range | 0.049-2.5 mmol/L |
Recovery Rate | 103 |
Inter CV | 8.1 |