Intrument | Spectrophotometer(660 nm) |
Assay Time | 60 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,tissue |
Synonyms | Pi |
Detection Principal | Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm. |
Reagents | Normal saline (0.9% NaCl) |
Labware | Water bath, Centrifuge, Micropipettor, Vortex mixer |
Size | 100Assays |
Sensitivity | 0.005 mmol/L |
Detection Range | 0.005-2.0 mmol/L |
Recovery Rate | 102 |
Inter CV | 1.3 |