| Intrument | Spectrophotometer(520 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,saliva,urine |
| Synonyms | BUN |
| Detection Principal | In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows: |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Vortex mixer, Micropipettor, Incubator? Water bath |
| Size | 100Assays |
| Sensitivity | 0.12 mmol/L |
| Detection Range | 0.12-15 mmol/L |
| Recovery Rate | 101 |
| Inter CV | 9.9 |