| Intrument | Spectrophotometer(715 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,animal tissue,cells |
| Synonyms | NEFA |
| Detection Principal | Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm. |
| Labware | Vortex mixer, Micropipettor |
| Size | 100Assays |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-2.0 mmol/L |
| Recovery Rate | 100 |
| Inter CV | 6.1 |