Intrument | Spectrophotometer(340 nm) |
Assay Time | 80 min |
Validity | 3 |
Assay Type | Enzyme Activity |
Sample Type | Serum,plasma,animal tissue,cells |
Synonyms | CK,Creatine phosphokinase |
Detection Principal | Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm. |
Reagents | PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Incubator, Vortex mixer |
Size | 100Assays |
Sensitivity | 3.7 U/L |
Detection Range | 3.7-160 U/L |
Inter CV | 8.1 |