| Intrument | Spectrophotometer(340 nm) |
| Assay Time | 80 min |
| Validity | 3 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,animal tissue,cells |
| Synonyms | CK,Creatine phosphokinase |
| Detection Principal | Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm. |
| Reagents | PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Incubator, Vortex mixer |
| Size | 100Assays |
| Sensitivity | 3.7 U/L |
| Detection Range | 3.7-160 U/L |
| Inter CV | 8.1 |