| Intrument | Microplate reader(690-730 nm,optimum wavelength: 715 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Animal tissue,cells |
| Synonyms | NEFA |
| Detection Principal | Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm. |
| Labware | Vortex mixer, Micropipettor, Multichannel transferpettor (300 ?L) |
| Size | 96T |
| Sensitivity | 0.15 mmol/L |
| Detection Range | 0.15-1.5 mmol/L |
| Recovery Rate | 101 |
| Inter CV | 5.1 |