Intrument | Microplate reader(530-540 nm,optimum wavelength: 532 nm) |
Assay Time | 65 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,animal tissue |
Synonyms | MDA |
Detection Principal | MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm. |
Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ?99.5%), Absolute ethanol |
Labware | Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers |
Size | 96T |
Sensitivity | 1.13 ?mol/L |
Detection Range | 2.92-40 ?mol/L |
Recovery Rate | 97.8 |
Inter CV | 7.2 |