Intrument | Microplate reader(400-410 nm,optimum wavelength: 405 nm) |
Assay Time | 40 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum,plasma,cells,cell culture supernatant,tissue |
Synonyms | CAT |
Detection Principal | The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm. |
Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex Mixer, Incubator |
Size | 96T |
Sensitivity | 1.12 U/mL |
Detection Range | 1.12 -150 U/mL |
Recovery Rate | 100 |
Inter CV | 7.7 |