Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Cat No.:HR3BC1201
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Microplate reader(400-420 nm,optimum wavelength: 412 nm)
Assay Time 40 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,cells,cell culture supernatant,tissue
Synonyms GSH-PX
Detection Principal Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Incubator, Vortex mixer, Centrifuge
Sensitivity 17.17 U
Detection Range 17.17-518.32 U
Recovery Rate 104
Inter CV 6.2

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