| Intrument | Fluorescent Microplate reader(?ex/em = 520 nm/550 nm) |
| Assay Time | 80 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,animal tissue,cells |
| Synonyms | TBARS |
| Detection Principal | TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm. |
| Reagents | Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Vortex mixer, Water bath, Micropipettor |
| Size | 96T |
| Sensitivity | 0.09 ?mol/L |
| Detection Range | 0.09-10 ?mol/L |
| Recovery Rate | 95.7 |
| Inter CV | 2.8 |