CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Cat No.:HR3BC1205
Product available

  Product info

Detection Method: Colorimetric method

Storage : Reagent 4: -20?, others: 2-8?
Product Details

Intrument Microplate reader(530-570 nm,optimum wavelength: 550 nm)
Assay Time 70 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,urine,cells,cell culture supernatant,tissue
Synonyms CuZn-SOD,Mn-SOD,T-SOD
Detection Principal Superoxide anion (O2?-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2?-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
ReagentsNormal saline (0.9% NaCl)
LabwareMicropipettor, Centrifuge, Incubator, Vortex mixer
Sensitivity 1.35 U/mL
Detection Range 1.35-62 U/mL
Recovery Rate 99
Inter CV 9.6

Write us

Superfast Delivery
Customer Support
Secure Online Payment
website designing in delhi website development company in delhi website developers in delhi freelancer website designer in delhi india best website development company in delhi