| Intrument | Microplate reader(530-570 nm,optimum wavelength: 550 nm) |
| Assay Time | 70 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,urine,cells,cell culture supernatant,tissue |
| Synonyms | CuZn-SOD,Mn-SOD,T-SOD |
| Detection Principal | Superoxide anion (O2?-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2?-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 1.35 U/mL |
| Detection Range | 1.35-62 U/mL |
| Recovery Rate | 99 |
| Inter CV | 9.6 |